The combinatorial barcoding and targeted chromatin release (CoBATCH) uses the same strategy (combinatorial indexing) and oligo design as sci-ATAC-seq. This method is kind of a combination of sci-ATAC-seq and CUT&TAG, where they used a protein A-Tn5 fusion protein to tether the pA-Tn5 to specific genomic loci bound by proteins of interest using antibodies.

For a brief introduction and backgound about the related methods, I have written a blog post. Click here to have a look!

Since CoBATCH uses the same oligo design as sci-ATAC-seq, for the step-by-step library generation, it is the same as sci-ATAC-seq. Click the sci-ATAC-seq page for details.